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Passive immunization of rainbow trout with chicken immunoglobins (IGY) Arasteh, Nikoo
Abstract
Passive immunization as an alternative to vaccination or antibiotic therapy, involves use of a pathogen specific antibody raised in other animals to provide extended disease resistance to the host animals or humans. The egg yolks of hens are a rich source of specific immunoglobulins (IgY) which can be easily extracted and incorporated into the human or animal diets. However, an effective method of IgY delivery into the host animal system is needed. In this study, enhancement of rainbow trout resistance against Vibrio anguillarum infection has been used as a model to investigate passage of IgY through the gut barrier into the bloodstream and the passive immunization that pathogen specific IgY may confer. High titers of anti-V. anguillarum IgY were raised in vaccinated hens, recovered from the water-soluble fraction (WSF) of the egg-yolks and subsequently used in intraperitoneal (IP) injection, oral intubation or feeding of the trout. Western blotting of such IgY revealed a strong reactivity with V. anguillarum whole cell lysate and lipopolysaccharide, which was as strong as that of the rabbit IgG and stronger than that of the fish IgM. Immunological properties of IgY as measured by ELISA were not affected by freeze-drying, vacuum microwave drying, air-drying, or spray drying. IP injected anti- Vibrio IgY was transferred into the fish system in high enough levels to confer protection against Vibriosis in an experimental challenge. This protective effect which was retained at least 14 days post IgY injection, proved efficacy of pathogen-specific IgY in enhancement of disease resistance. To investigate absorption through trout digestive tract, IgY was intubated both anally and orally at the levels of 0.1mg and 1.4-2.7mg fish-1, respectively. Under the conditions of this study, anally intubated IgY (0.1mg) did not appear in the serum in a detectable level. Oral intubation of WSF led to absorption of IgY in an immunologically active form; however the levels were 800 to 2500 times lower than those resulting from IP injection. Among the detergents co-administered with IgY, deoxycholate, Mega9 and octyl-β-glucoside mediated the highest enhancement of IgY absorption. Use of Mega9 raised serum IgY to levels only 12 to 18 times lower than the levels after IP injection o f a similar dose. Encapsulation of WSF in polylactide-co-glycolide did not improve IgY uptake. Oral administration of anti-Vibrio IgY in co-delivery with detergents resulted in different levels of protection of rainbow trout against Vibriosis following an immersion challenge, which in some cases was comparable to the protection offered by IP injection of IgY. The efficacy of continued feeding of specific IgY before and after exposure to the pathogenic bacteria has yet to be explored.
Item Metadata
Title |
Passive immunization of rainbow trout with chicken immunoglobins (IGY)
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2000
|
Description |
Passive immunization as an alternative to vaccination or antibiotic therapy,
involves use of a pathogen specific antibody raised in other animals to provide extended
disease resistance to the host animals or humans. The egg yolks of hens are a rich source
of specific immunoglobulins (IgY) which can be easily extracted and incorporated into
the human or animal diets. However, an effective method of IgY delivery into the host
animal system is needed.
In this study, enhancement of rainbow trout resistance against Vibrio anguillarum
infection has been used as a model to investigate passage of IgY through the gut barrier
into the bloodstream and the passive immunization that pathogen specific IgY may
confer. High titers of anti-V. anguillarum IgY were raised in vaccinated hens, recovered
from the water-soluble fraction (WSF) of the egg-yolks and subsequently used in
intraperitoneal (IP) injection, oral intubation or feeding of the trout. Western blotting of
such IgY revealed a strong reactivity with V. anguillarum whole cell lysate and
lipopolysaccharide, which was as strong as that of the rabbit IgG and stronger than that of
the fish IgM.
Immunological properties of IgY as measured by ELISA were not affected by
freeze-drying, vacuum microwave drying, air-drying, or spray drying. IP injected anti-
Vibrio IgY was transferred into the fish system in high enough levels to confer protection
against Vibriosis in an experimental challenge. This protective effect which was retained
at least 14 days post IgY injection, proved efficacy of pathogen-specific IgY in
enhancement of disease resistance.
To investigate absorption through trout digestive tract, IgY was intubated both
anally and orally at the levels of 0.1mg and 1.4-2.7mg fish-1, respectively. Under the
conditions of this study, anally intubated IgY (0.1mg) did not appear in the serum in a
detectable level. Oral intubation of WSF led to absorption of IgY in an immunologically
active form; however the levels were 800 to 2500 times lower than those resulting from
IP injection. Among the detergents co-administered with IgY, deoxycholate, Mega9 and
octyl-β-glucoside mediated the highest enhancement of IgY absorption. Use of Mega9
raised serum IgY to levels only 12 to 18 times lower than the levels after IP injection o f a
similar dose. Encapsulation of WSF in polylactide-co-glycolide did not improve IgY
uptake. Oral administration of anti-Vibrio IgY in co-delivery with detergents resulted in
different levels of protection of rainbow trout against Vibriosis following an immersion
challenge, which in some cases was comparable to the protection offered by IP injection
of IgY. The efficacy of continued feeding of specific IgY before and after exposure to
the pathogenic bacteria has yet to be explored.
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Extent |
23765076 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-07-21
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0089669
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2000-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.