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Analysis of Y-linked temperature-sensitive mutations in Drosophila melanogaster causing male sterility or great aspirations Suchowersky, Oksana
Abstract
A number of EMS-induced heat-sensitive and cold-sensitive male sterile mutations were recovered on an XY chromosome. All mutations which recovered fertility in the presence of a free Y chromosome were classified as Y-linked. Of the Y- linked hs mutations, all were fertile with a free Y[sup L] fragment, while the cs mutations were fertile with a Y[sup S] fragment. Ten of the hs Y-linked mutations were studied in detail; eight of these were shown to be unambiguously located on Y[sup L], and had a temperature sensitive period coinciding with spermatid differentiation. Two of the hs mutant stocks were double mutations and at least one of these stocks had an X-linked mutation which interacted with the Y chromosome. This stock had a tsp which, although exerting its effect during spermio- genesis, was prior to that of the mutations located on Y[sup L]. Of the 5 cs mutations classified as Y-linked, at least three were shown to be mutations on the X chromosome which interacted with Y[sup S] The tsp's of these mutations are either pre-or early post-meiotic. Thus, no ts mutations were recovered on Y[sup S]. These results suggest that: 1) the fertility factors on Y[sup L] are present in single copies and produce products which are directly involved in spermiogenesis, 2) the genes on Y[sup S] could have a regulatory function during spermatogenesis, and either do not produce products which are thermo-labile, or are present in multiple copies, 3) there are a class of genes on the X chromosome which affect spermatogenesis through interaction with the Y chromosome. Several models were proposed to account for this X-Y chromosome interaction.
Item Metadata
Title |
Analysis of Y-linked temperature-sensitive mutations in Drosophila melanogaster causing male sterility or great aspirations
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1975
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Description |
A number of EMS-induced heat-sensitive and cold-sensitive
male sterile mutations were recovered on an XY chromosome.
All mutations which recovered fertility in the presence of a
free Y chromosome were classified as Y-linked. Of the Y-
linked hs mutations, all were fertile with a free Y[sup L] fragment,
while the cs mutations were fertile with a Y[sup S] fragment.
Ten of the hs Y-linked mutations were studied in detail;
eight of these were shown to be unambiguously located on Y[sup L], and had a temperature sensitive period coinciding with spermatid differentiation. Two of the hs mutant stocks were double mutations and at least one of these stocks had an X-linked mutation
which interacted with the Y chromosome. This stock
had a tsp which, although exerting its effect during spermio-
genesis, was prior to that of the mutations located on Y[sup L].
Of the 5 cs mutations classified as Y-linked, at least
three were shown to be mutations on the X chromosome which
interacted with Y[sup S] The tsp's of these mutations are either
pre-or early post-meiotic. Thus, no ts mutations were
recovered on Y[sup S].
These results suggest that:
1) the fertility factors on Y[sup L] are present in single copies
and produce products which are directly involved in spermiogenesis,
2) the genes on Y[sup S] could have a regulatory function during spermatogenesis, and either do not produce products which are thermo-labile, or are present in multiple copies,
3) there are a class of genes on the X chromosome which affect spermatogenesis through interaction with the Y chromosome. Several models were proposed to account for this X-Y chromosome interaction.
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Genre | |
Type | |
Language |
eng
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Date Available |
2010-01-28
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0093248
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.