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Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion

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Title: Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion
Author: Hansen, Christine S.
Degree Master of Science - MSc
Program Food Science
Copyright Date: 1988
Subject Keywords Yeast; Protoplasts; Carotenoids; Yeasts; Protoplasts; Carotenoids
Abstract: Protoplasts were prepared from two yeast strains P. rhodozyma (ATCC 24202) and K. fragilis (ATCC 8455). Protoplasts prepared from P. rhodozyma were facilitated by prior growth of the cells in a media containing S-(2-aminoethyl)-L-cysteine. Protoplasts from these two yeast genera were fused either by the use of electrofusion or polyethylene glycol treatment. Stable carotenoid producing cell lines were selected by growth at 30°C on yeast nitrogen base plus galactose. Selected single fusants display taxonomic characteristics common to both genera with a cellular morphology and a carotenoid composition similar to that of P. rhodozyma.
URI: http://hdl.handle.net/2429/27935
Series/Report no. UBC Retrospective Theses Digitization Project [http://www.library.ubc.ca/archives/retro_theses/]
Scholarly Level: Graduate

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