Go to  Advanced Search

Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion

Show full item record

Files in this item

Files Size Format Description   View
UBC_1988_A6_7 H36_5.pdf 5.773Mb Adobe Portable Document Format   View/Open
 
Title: Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion
Author: Hansen, Christine S.
Degree: Master of Science - MSc
Program: Food Science
Copyright Date: 1988
Subject Keywords Yeast;Protoplasts;Carotenoids;Yeasts;Protoplasts;Carotenoids
Issue Date: 2010-08-30
Publisher University of British Columbia
Series/Report no. UBC Retrospective Theses Digitization Project [http://www.library.ubc.ca/archives/retro_theses/]
Abstract: Protoplasts were prepared from two yeast strains P. rhodozyma (ATCC 24202) and K. fragilis (ATCC 8455). Protoplasts prepared from P. rhodozyma were facilitated by prior growth of the cells in a media containing S-(2-aminoethyl)-L-cysteine. Protoplasts from these two yeast genera were fused either by the use of electrofusion or polyethylene glycol treatment. Stable carotenoid producing cell lines were selected by growth at 30°C on yeast nitrogen base plus galactose. Selected single fusants display taxonomic characteristics common to both genera with a cellular morphology and a carotenoid composition similar to that of P. rhodozyma.
Affiliation: Land and Food Systems, Faculty of
URI: http://hdl.handle.net/2429/27935
Scholarly Level: Graduate

This item appears in the following Collection(s)

Show full item record

UBC Library
1961 East Mall
Vancouver, B.C.
Canada V6T 1Z1
Tel: 604-822-6375
Fax: 604-822-3893