Somatic embryogenesis of lodgepole pine (Pinus contorta) and subalpine larch (Larix lyallii.) for conservation purposes

UBC Undergraduate Research

Somatic embryogenesis of lodgepole pine (Pinus contorta) and subalpine larch (Larix lyallii.) for conservation purposes Toon, Sandra M.

Abstract

This study was initiated with the aim of establishing somatic embryogenesis culture from lodgepole pine (for clonal propagation of superior genotypes with mountain pine beetle resistant genotypes) and subalpine larch (for conservation purposes), both of which have never been attempted prior. Cones were collected on Mt Frosty, Manning Provincial Park for larch and from Vernon Ministry of Forestry orchard (307) for lodgepole pine. Optimum stage of cone maturity for initiation success was tested by 4 collections, made at 1 week intervals for lodgepole pine and 3 collections made at 2 week intervals for subalpine larch. Three media were compared: modified Litvay medium, as standard and low and DCR. Pro-embryonal mass (PEM) from lodgepole pine developed as early as 4 weeks, which is twice as fast when compared to subalpine larch (8 weeks). Desired cone maturity was present in lodgepole pine collections 3 and 4, but never present for subalpine larch. In conclusion, no embryogenic tissue was obtained from either species.

Item Metadata

Title	Somatic embryogenesis of lodgepole pine (Pinus contorta) and subalpine larch (Larix lyallii.) for conservation purposes
Creator	Toon, Sandra M.
Date Issued	2010-04-12
Description	This study was initiated with the aim of establishing somatic embryogenesis culture from lodgepole pine (for clonal propagation of superior genotypes with mountain pine beetle resistant genotypes) and subalpine larch (for conservation purposes), both of which have never been attempted prior. Cones were collected on Mt Frosty, Manning Provincial Park for larch and from Vernon Ministry of Forestry orchard (307) for lodgepole pine. Optimum stage of cone maturity for initiation success was tested by 4 collections, made at 1 week intervals for lodgepole pine and 3 collections made at 2 week intervals for subalpine larch. Three media were compared: modified Litvay medium, as standard and low and DCR. Pro-embryonal mass (PEM) from lodgepole pine developed as early as 4 weeks, which is twice as fast when compared to subalpine larch (8 weeks). Desired cone maturity was present in lodgepole pine collections 3 and 4, but never present for subalpine larch. In conclusion, no embryogenic tissue was obtained from either species.
Subject	Somatic embryogenesis; Zygotic embryogenesis; Lodgepole pine; Alpine larch; Alpine ecosystems; Germination; Tissue culture
Genre	Graduating Project
Type	Text
Language	eng
Series	University of British Columbia. FRST 498
Date Available	2010-11-19
Provider	Vancouver : University of British Columbia Library
Rights	Attribution-NonCommercial-NoDerivatives 4.0 International
DOI	10.14288/1.0075614
URI	http://hdl.handle.net/2429/30045
Affiliation	Forestry, Faculty of
Campus	UBCV
Peer Review Status	Unreviewed
Scholarly Level	Undergraduate
Rights URI	http://creativecommons.org/licenses/by-nc-nd/4.0/
Aggregated Source Repository	DSpace

Open Collections

UBC Undergraduate Research