Go to  Advanced Search

Pre-processing of quantitative phenotypes from high throughput studies

Show full item record

Files in this item

Files Size Format Description   View
ubc_2007-0138a.pdf 7.365Mb Adobe Portable Document Format   View/Open
 
Title: Pre-processing of quantitative phenotypes from high throughput studies
Author: Kanters, Steve
Degree Master of Arts - MA
Program Statistics
Copyright Date: 2006
Abstract: High throughput phenotypic experiments include both deletion sets and R N A i experiments. They are genome wide and require much physical space. As a result, multiple plates are often required in order to cover the whole genome. The use of multiple plates leads to systematic plate-wise experimental artefact, which impede statistical inference. In this paper, current pre-processing methodology will be reviewed. Their fundamental principle is to align a common feature shared by all plates. From this very principle, we propose an improved method which simultaneously estimates all parameters required for the pre-processing transformation. Some of the alignment features popular today implicitly assume conditions which are often not met in practice. We discuss the various choices of features to align. Specifically, the upper quantiles and the mean of the left tail trimmings of each plate's data distribution are features which are always available and simple to obtain. Moreover, they are robust to non-randomization of genes to plates. Their use will be motivated through simulation and applied to real data. Applications to real data will be used to demonstrate superiority over current methods as well as to discuss choices in transformation types.
URI: http://hdl.handle.net/2429/31404
Series/Report no. UBC Retrospective Theses Digitization Project [http://www.library.ubc.ca/archives/retro_theses/]
Scholarly Level: Graduate

This item appears in the following Collection(s)

Show full item record

All items in cIRcle are protected by copyright, with all rights reserved.

UBC Library
1961 East Mall
Vancouver, B.C.
Canada V6T 1Z1
Tel: 604-822-6375
Fax: 604-822-3893