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Cytological alteration in the rat stomach postburn

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Title: Cytological alteration in the rat stomach postburn
Author: Harris, Curtis Norman
Degree: Master of Science - MSc
Program: Surgery
Copyright Date: 1972
Subject Keywords Gastric mucosa;Rats
Issue Date: 2011-04-28
Publisher University of British Columbia
Series/Report no. UBC Retrospective Theses Digitization Project [http://www.library.ubc.ca/archives/retro_theses/]
Abstract: Gastric mucosal erosions were induced in the glandular stomach of rats by scalding. The incorporation of Thymidine-methyl³-H into desoxyribonucleic acid was used to determine changes in gastric epithelial cell proliferating ability. Total desoxyribonucleic acid per milligram of gastric tissue was also determined. Sampling was done at twenty-four hours, seven days, and fifteen days postburn. Eighty-nine point two percent of rats with a standard 26.5 ± 2% scald burn had developed gastric mucosal erosions by twenty-four hours postburn. Seventeen point eight percent of burned rats had erosions by seven days and the incidence rose to 46.4% by fifteen days postburn. Ten point three percent of control rats in all sampling periods developed erosions. The total desoxyribonucleic acid in the gastric samples did not change significantly in any treatment period nor was it changed by treatment. Uptake of thymidine-methyl³-H was depressed twenty-four hours postburn and renewed so through seven days postburn. The results at the fifteen day sampling were inconclusive. By light microscope, the gastric surface epithelium was lifted from the lamina propria and at times there was complete denudation of this cell layer. The rat is a satisfactory animal model for gross study of mucosal erosions to at least fifteen day postburn. Because of eschar cannibalization inducing variable secretory status, the rat model was not suitable for thymidine uptake studies past seven days postburn.
Affiliation: Medicine, Faculty of
URI: http://hdl.handle.net/2429/34099
Scholarly Level: Graduate

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