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The characterization of atm-1 in Caenorhabditis elegans

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Title: The characterization of atm-1 in Caenorhabditis elegans
Author: Huang, Chin
Degree: Master of Science - MSc
Program: Medical Genetics
Copyright Date: 2011
Issue Date: 2011-06-08
Publisher University of British Columbia
Abstract: Loss of function of ATM (ataxia-telangiectasia, mutated) was discovered to be the genetic cause of the human disorder Ataxia-Telangiectasia (A-T). A-T is a rare, autosomal recessive human disorder that presents with multiple symptoms, including ataxia, telangiectasia, neurodegeneration, immunodeficiency, radiosensitivity, genomic instability, and a predisposition to developing cancer. The predicted ATM orthologue in Caenorhabditis elegans (C. elegans) is the gene atm-1. C. elegans is a model system that is easily amendable to molecular and genetic research. In this thesis, I have characterized the structure of atm-1 and examined its mutant phenotype. The predicted gene model for atm-1 was smaller than known orthologues. Using cDNA analysis and sequencing, I have shown that the three gene predictions atm-1, K10E9.1, and F56C11.4 make up the complete coding region. I observed differences from the original prediction including unpredicted splice sites and exonic sequences. A single atm-1 mutant allele, gk186, exists. I have shown that the gk186 deletion produces two alternative transcripts, one of which retains the protein kinase domain. Thus, in contrast to previous expectations that this mutation generated a loss of function phenotype, it is likely that it produces a protein with kinase function. The phenotype of the mutant is radiation sensitivity, as expected, but is not as sensitive as brd-1, another loss of function mutant in the same double strand break repair pathway. This result is consistent with partial function retention of atm-1. In addition, mutant atm-1 showed chromosomal instability in X-chromosome loss and subsequent sterility. Some of the mutational events were captured as lethals using the eT1 balancer system documenting a mutator phenotype of atm-1. Characterization of atm-1 gene function in C. elegans may provide additional information about its function in other organisms including humans.
Affiliation: Medicine, Faculty of
URI: http://hdl.handle.net/2429/35326
Scholarly Level: Graduate

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