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An action spectrum apparatus

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Title: An action spectrum apparatus
Author: Brooks, Donald Elliott
Degree Master of Science - MSc
Program Physics
Copyright Date: 1967
Subject Keywords Carbon monoxide; Instrumental analysis; Scientific apparatus and instruments
Abstract: An instrument is described which is capable of measuring the action spectrum of the removal of CO inhibition of respiration by light. In the method employed here, a cell suspension in a CO-O₂ atmosphere is alternately exposed to two wavelengths of light. Their photochemical effects are balanced using an 0₂ electrode as the null detector. The light intensities at the balance points from a series of wavelength pairs are used to determine the ratios of the extinction coefficients of the CO - oxidase complex, at the various wavelengths, to the extinction coefficient at a standard wavelength. An action spectrum for Bakers yeast is shown.
URI: http://hdl.handle.net/2429/36327
Series/Report no. UBC Retrospective Theses Digitization Project [http://www.library.ubc.ca/archives/retro_theses/]
Scholarly Level: Graduate

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