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The effect of micromachined surfaces on osteogenesis in vitro Ratkay, Julia
Abstract
Since the use of osseointegrated implants became common in both dentistry and orthopaedics, researchers have been investigating the problem of improving stabilization of the implant. It is widely accepted that the solution lies in improving the biomaterial interface. Implants with various surface designs and chemical compositions have been investigated at the experimental and clinical levels. Micromachining, a technique widely used i n microelectronics, has the capability to produce surface topographies with precise dimensions and high reproducibility. This allows the testing of the effect of implant surface topography on osseointegration in controlled experiments. These experiments aimed to test the ability of micromachined surfaces to promote bone formation in vitro. A recently developed osteogenic culture was optimized and characterized, in which rat calvarial cells were cultured in the presence of ascorbic acid and organic phosphate in the medium. This method provided a reliable model of reproducible mineralized tissue formation. Histochemical methods, TEM, S EM and EDX techniques demonstrated that the mineralized tissue formed after two weeks culturing had an elevated alkalinephosphatase activity, was rich in collagen, and had a structure, similar to the original calvarial bone. The ability of micromachined surfaces to facilitate osteogenesis was studied when these osteogenic cultures were grown on grooved, pitted, and smooth micromachined surfaces. The developed mineralized tissue was stained utilizing von Kossa's technique and its amount quantified with an UltroScan X L Laser Densitometer. The area, shape and orientation of the mineralized bone colonies were measured with a Videoplan Image Analysis System (Zeiss). There was a statistically significant difference of bone formation between the micromachined and smooth surfaces. Bone-like tissue formed to the greatest extent on pitted surfaces, and to a lesser extent on grooves, but few bone nodules were found on the smooth control surfaces. The colonies on the grooved surfaces were elongated and followed the direction of the grooves, while those on the smooth and pitted surfaces were rounder and randomly oriented. The results indicate that bone-like tissue formation is influenced by the substratum surface topography and micromachining may be useful i n determining the ideal surface topography for promoting bone formation on titanium surfaces.
Item Metadata
Title |
The effect of micromachined surfaces on osteogenesis in vitro
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1995
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Description |
Since the use of osseointegrated implants became common in both
dentistry and orthopaedics, researchers have been investigating the problem of
improving stabilization of the implant. It is widely accepted that the solution
lies in improving the biomaterial interface. Implants with various surface
designs and chemical compositions have been investigated at the experimental
and clinical levels. Micromachining, a technique widely used i n
microelectronics, has the capability to produce surface topographies with
precise dimensions and high reproducibility. This allows the testing of the
effect of implant surface topography on osseointegration in controlled
experiments.
These experiments aimed to test the ability of micromachined surfaces to
promote bone formation in vitro. A recently developed osteogenic culture was
optimized and characterized, in which rat calvarial cells were cultured in the
presence of ascorbic acid and organic phosphate in the medium. This method
provided a reliable model of reproducible mineralized tissue formation.
Histochemical methods, TEM, S EM and EDX techniques demonstrated that the
mineralized tissue formed after two weeks culturing had an elevated alkalinephosphatase
activity, was rich in collagen, and had a structure, similar to the
original calvarial bone.
The ability of micromachined surfaces to facilitate osteogenesis was
studied when these osteogenic cultures were grown on grooved, pitted, and
smooth micromachined surfaces. The developed mineralized tissue was stained
utilizing von Kossa's technique and its amount quantified with an UltroScan X L
Laser Densitometer. The area, shape and orientation of the mineralized bone
colonies were measured with a Videoplan Image Analysis System (Zeiss).
There was a statistically significant difference of bone formation between the
micromachined and smooth surfaces. Bone-like tissue formed to the greatest
extent on pitted surfaces, and to a lesser extent on grooves, but few bone
nodules were found on the smooth control surfaces. The colonies on the grooved
surfaces were elongated and followed the direction of the grooves, while those
on the smooth and pitted surfaces were rounder and randomly oriented.
The results indicate that bone-like tissue formation is influenced by the
substratum surface topography and micromachining may be useful i n
determining the ideal surface topography for promoting bone formation on
titanium surfaces.
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Extent |
9628210 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-01-17
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0086739
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1995-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.