- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Solid state NMR investigation of protein-based biomaterials....
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Solid state NMR investigation of protein-based biomaterials. Resilin : an extremely efficient elastomeric protein Reddin, Andrew L. C.
Abstract
Solid state nuclear magnetic resonance experiments were performed in order to investigate the microscopic properties of three resilin/resilin-like proteins: An16, rec1-resilin, and natural resilin in dragonfly tendons. Three different types of experiments were performed: measurements of chemical shifts in carbon-13 spectra, measurements of residual quadrupole couplings in deuterated water absorbed in the samples, and measurements of proton residual dipole couplings based on the buildup of multiple quantum coherences. The results suggest that the molecular chains in the materials tested are primarily randomly coiled and lacking in regular structure, and are able to easily change between many transient conformations. These conformations can vary significantly in terms of their structural characteristics, resulting in a broad distribution of localized dynamics. When stretched, An16 showed a slightly increased tendency to adopt beta-sheet secondary structure. The natural resilin also exhibited slightly more rigid structure than the other materials, which may be related to greater efficiency in the natural crosslinking process.
Item Metadata
Title |
Solid state NMR investigation of protein-based biomaterials. Resilin : an extremely efficient elastomeric protein
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
2012
|
Description |
Solid state nuclear magnetic resonance experiments were performed in order to investigate the microscopic properties of three resilin/resilin-like proteins: An16, rec1-resilin, and natural resilin in dragonfly tendons. Three different types of experiments were performed: measurements of chemical shifts in carbon-13 spectra, measurements of residual quadrupole couplings in deuterated water absorbed in the samples, and measurements of proton residual dipole couplings based on the buildup of multiple quantum coherences. The results suggest that the molecular chains in the materials tested are primarily randomly coiled and lacking in regular structure, and are able to easily change between many transient conformations. These conformations can vary significantly in terms of their structural characteristics, resulting in a broad distribution of localized dynamics. When stretched, An16 showed a slightly increased tendency to adopt beta-sheet secondary structure. The natural resilin also exhibited slightly more rigid structure than the other materials, which may be related to greater efficiency in the natural crosslinking process.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2012-04-23
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution-NonCommercial-ShareAlike 3.0 Unported
|
DOI |
10.14288/1.0085662
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
2012-05
|
Campus | |
Scholarly Level |
Graduate
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-ShareAlike 3.0 Unported