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Detection of genetic variation among Endocronartium harknessii populations in British Columbia using rDNA RFLPS and RAPD markers Sun, Li-Juan
Abstract
DNA-level variability in Endocronartium harknessii (Moore) Hiratsuka, the cause of western gall rust On lodgepole pine (Pinus contorta Dougl.), was assessed using RAPD's and RFLP's. The material analyzed consisted of aeciospores from 120 wild isolates collected at 12 geographically separate locations (10 galls each) ranging from the interior (6 locations) to the coast (4 locations) of the province of British Columbia with two collections from Manning Park, representing one of the passes between these regions. Using the RAPD technique, of 180 primers screened, 64 yielded clear polymorphic bands. Of these 13 were chosen for the main study. These 13 primers yielded 96 polymorphic bands. All galls exhibited a unique RAPD-type. Principle Component Analysis (PCA) revealed that coastal and interior collections were quite distinct, with the exception of two adjacent interior collections (Quesnel and Ten Mile Lake). On the other hand, of the two collections from Manning Park, the high elevation one was clearly related to the interior population, while the low elevation one was clearly coastal indicating that there is probably limited gene flow between the two regions. Dendrograms constructed, using unweighted pair group arithmetic mean analysis (UPGMA) based on similarity measure for individual galls separated most local collections into distinct clusters, and revealed relationships between local collections similar to PCA. The RFLP technique was used for a small sample of galls and revealed considerable variation between them. However, since this technique was rather laborious and required larger amounts of DNA than could be extracted from spores produced by small, young, clean galls, it was not suitable for a large population study. Analysis of DNA from different sectors of large old galls using RAPD's showed some polymorphisms between sectors. The origin of these polymorphisms is not clear, A species-specific dsRNA was detected in several galls, as well as in collections of related Cronartium rusts, suggesting the presence of a mycovirus in these rust populations.
Item Metadata
Title |
Detection of genetic variation among Endocronartium harknessii populations in British Columbia using rDNA RFLPS and RAPD markers
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1996
|
Description |
DNA-level variability in Endocronartium harknessii (Moore) Hiratsuka, the cause
of western gall rust On lodgepole pine (Pinus contorta Dougl.), was assessed using
RAPD's and RFLP's. The material analyzed consisted of aeciospores from 120 wild
isolates collected at 12 geographically separate locations (10 galls each) ranging from the
interior (6 locations) to the coast (4 locations) of the province of British Columbia with
two collections from Manning Park, representing one of the passes between these regions.
Using the RAPD technique, of 180 primers screened, 64 yielded clear polymorphic bands.
Of these 13 were chosen for the main study. These 13 primers yielded 96 polymorphic
bands. All galls exhibited a unique RAPD-type.
Principle Component Analysis (PCA) revealed that coastal and interior collections
were quite distinct, with the exception of two adjacent interior collections (Quesnel and
Ten Mile Lake). On the other hand, of the two collections from Manning Park, the high
elevation one was clearly related to the interior population, while the low elevation one
was clearly coastal indicating that there is probably limited gene flow between the two
regions.
Dendrograms constructed, using unweighted pair group arithmetic mean analysis
(UPGMA) based on similarity measure for individual galls separated most local collections
into distinct clusters, and revealed relationships between local collections similar to PCA.
The RFLP technique was used for a small sample of galls and revealed
considerable variation between them. However, since this technique was rather laborious
and required larger amounts of DNA than could be extracted from spores produced by
small, young, clean galls, it was not suitable for a large population study. Analysis of DNA from different sectors of large old galls using RAPD's showed
some polymorphisms between sectors. The origin of these polymorphisms is not clear,
A species-specific dsRNA was detected in several galls, as well as in collections of
related Cronartium rusts, suggesting the presence of a mycovirus in these rust
populations.
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Extent |
6271375 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-02-06
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0075236
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1996-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.