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Ultrafast microfluidic droplet sorter extension work Kong, Luke; Wu, Samuel
Abstract
This project builds on work done by a previous APSC 459 group, who developed fluorescence- based droplet sorting device similar to that described by Agresti et al. up to the point where it could detect the fluorescence of droplets up to a rate of 1kHz. However, it was unable to sort droplets; this project sought to add droplet sorting functionality to their microfluidic chip design. Our project aimed to demonstrate droplet actuation with use of the existing experimental setup. This consisted of three main objectives: 1. Model effect of electrode design and geometry on droplet actuation using finite-‐ element analysis (implemented in COMSOL). 2. Demonstrate actuation of droplets, redesigning microfluidic chip if necessary. 3. Optimize setup towards droplet sorting at speeds on the same order (1-2 kHz) as exisiting work (Agresti, 2010). Using COMSOL we determined the factors that will most strongly affect the dielectrophoretic force on a droplet in a channel. From these results we redesigned the droplet sorter chip from the previous group and fabricated chips with a new electrode design (microfluidic channels to be filled with low-melting-point alloy). We then went on to demonstrate droplet redirection at a rate of at least 100Hz. Although droplet redirection, the main focus of this project, was successfully demonstrated, much work remains to be done on this project. The redirection needs to be coupled to the previous 459 group’s droplet detection setup in order for controlled droplet actuation. Recommendations were made about electrode fabrication, droplet transfer from generator to sorter and high voltage switching.
Item Metadata
Title |
Ultrafast microfluidic droplet sorter extension work
|
Creator | |
Date Issued |
2012-04-02
|
Description |
This
project
builds
on
work
done
by
a
previous
APSC
459
group,
who
developed
fluorescence-
based
droplet
sorting
device
similar
to
that
described
by
Agresti
et
al.
up
to
the
point
where
it
could
detect
the
fluorescence
of
droplets
up
to
a
rate
of
1kHz.
However,
it
was
unable
to
sort
droplets;
this
project
sought
to
add
droplet
sorting
functionality
to
their
microfluidic
chip
design.
Our
project
aimed
to
demonstrate
droplet
actuation
with
use
of
the
existing
experimental
setup.
This
consisted
of
three
main
objectives:
1. Model
effect
of
electrode
design
and
geometry
on
droplet
actuation
using
finite-‐
element
analysis
(implemented
in
COMSOL).
2. Demonstrate
actuation
of
droplets,
redesigning
microfluidic
chip
if
necessary.
3. Optimize
setup
towards
droplet
sorting
at
speeds
on
the
same
order
(1-2
kHz)
as
exisiting
work
(Agresti,
2010).
Using
COMSOL
we
determined
the
factors
that
will
most
strongly
affect
the
dielectrophoretic
force
on
a
droplet
in
a
channel.
From
these
results
we
redesigned
the
droplet
sorter
chip
from
the
previous
group
and
fabricated
chips
with
a
new
electrode
design
(microfluidic
channels
to
be
filled
with
low-melting-point
alloy).
We
then
went
on
to
demonstrate
droplet
redirection
at
a
rate
of
at
least
100Hz.
Although
droplet
redirection,
the
main
focus
of
this
project,
was
successfully
demonstrated,
much
work
remains
to
be
done
on
this
project.
The
redirection
needs
to
be
coupled
to
the
previous
459
group’s
droplet
detection
setup
in
order
for
controlled
droplet
actuation.
Recommendations
were
made
about
electrode
fabrication,
droplet
transfer
from
generator
to
sorter
and
high
voltage
switching.
|
Genre | |
Type | |
Language |
eng
|
Series | |
Date Available |
2012-09-20
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
DOI |
10.14288/1.0074470
|
URI | |
Affiliation | |
Campus | |
Peer Review Status |
Unreviewed
|
Scholarly Level |
Undergraduate
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International