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UBC Theses and Dissertations
Investigation of kalilo-like plasmids in Neurospora and Gelasinospora He, Cynthia Yingxin
Abstract
Mitochondrial DNA plasmids sharing great sequence homology with the senescenceinducing kalilo plasmid have been identified in natural isolates of Neurospora crassa, Neurospora intermedia, Neurospora tetrasperma, Neurospora discreta and a Neurospora-related genus Gelasinospora. Restriction enzyme analysis and sequence analysis have revealed that these plasmids are closely related by descent from a common ancestral plasmid. The phylogenetic tree constructed on the basis of the terminal inverted repeat sequences of the kalilo-like plasmids correlates well with the established taxonomy of their host fungi. The attempt to transfer kalilo-like plasmids to standard lab strains of N. crassa has had limited success. Only a few transferrants were obtained and they all proved to be unstable. Therefore, the comparison of plasmid function can not yet be made at the plasmid level with identical mitochondrial DNA and nuclear DNA background. However, the comparison of plasmid TIR sequences reveals some interesting features which may be relevant to the plasmid function. The failure of plasmid transfer indicates that horizontal transfer must be a rare event, though it may be evolutionarily significant as to contribute to the current distribution pattern of kalilo-related plasmids.
Item Metadata
Title |
Investigation of kalilo-like plasmids in Neurospora and Gelasinospora
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1996
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Description |
Mitochondrial DNA plasmids sharing great sequence homology with the senescenceinducing
kalilo plasmid have been identified in natural isolates of Neurospora crassa,
Neurospora intermedia, Neurospora tetrasperma, Neurospora discreta and a Neurospora-related
genus Gelasinospora. Restriction enzyme analysis and sequence analysis have revealed that
these plasmids are closely related by descent from a common ancestral plasmid. The
phylogenetic tree constructed on the basis of the terminal inverted repeat sequences of the
kalilo-like plasmids correlates well with the established taxonomy of their host fungi. The
attempt to transfer kalilo-like plasmids to standard lab strains of N. crassa has had limited
success. Only a few transferrants were obtained and they all proved to be unstable. Therefore,
the comparison of plasmid function can not yet be made at the plasmid level with identical
mitochondrial DNA and nuclear DNA background. However, the comparison of plasmid TIR
sequences reveals some interesting features which may be relevant to the plasmid function. The
failure of plasmid transfer indicates that horizontal transfer must be a rare event, though it may
be evolutionarily significant as to contribute to the current distribution pattern of kalilo-related
plasmids.
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Extent |
3426968 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-02-14
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0099068
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1996-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.