- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Characterization and cloning of suppressor of chs3-2D...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Characterization and cloning of suppressor of chs3-2D in Arabidopsis Zhu, Chipan
Abstract
Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN (NOD)-LIKE RECEPTOR (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high PATHOGENESIS-RELATED (PR) gene expression, and salicylic acid accumulation. To search for novel regulators involved in the CHS3-mediated immune signaling pathway, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. This suggests that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located in the Arabidopsis genome adjacent to CHS3 was found to be required for CHS3-mediated signaling. CSA1 is located 3.9kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.
Item Metadata
Title |
Characterization and cloning of suppressor of chs3-2D in Arabidopsis
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
2015
|
Description |
Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN (NOD)-LIKE RECEPTOR (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high PATHOGENESIS-RELATED (PR) gene expression, and salicylic acid accumulation. To search for novel regulators involved in the CHS3-mediated immune signaling pathway, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. This suggests that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located in the Arabidopsis genome adjacent to CHS3 was found to be required for CHS3-mediated signaling. CSA1 is located 3.9kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2015-08-31
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution-NonCommercial-NoDerivs 2.5 Canada
|
DOI |
10.14288/1.0167123
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
2015-05
|
Campus | |
Scholarly Level |
Graduate
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivs 2.5 Canada