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- NMR imaging and spectroscopy in cyrobiology
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NMR imaging and spectroscopy in cyrobiology Isbell, Stephanie A.
Abstract
Microscopic Chemical Shift Specific Slice Selective (C45) NMR imaging is demonstrated to be a nondestructive and noninvasive technique for monitoring the spatial distributions of solvents and of freezing/thawing phenomena. Studies of test samples show that the C4S NMR imaging sequence can yield quantitative maps of distributions of water., and dimethyl sulfoxide (DMSO). The unique characteristics of C4S NMR imaging are potentially very useful in studies of organ cryopreservation in which cryoprotective solvents (CPSs) like DMSO successfully prevent freezing damage to cells in suspension but presently cannot be used to preserve whole organs at subzero temperatures. There are no other techniques available which are capable of monitoring CPS and water concentration distributions as well as structural changes in organs during cryopreservation procedures. C4S NMR imaging is shown to be useful for monitoring the diffusion of the cryoprotectant DMSO into rat kidney and liver tissues. A detailed investigation of the possibility of mapping concentration distributions of DMSO and water at various temperatures using images with short repetition times verifies that DMSO and water in tissues can be quantified from image signal intensities within certain concentration ranges. Diffusion rates are easily obtained from the imaging data and are similar to those found in the literature. A simple method for determination of effective diffusion coefficients of DMSO in tissues is developed and shown to be more accurate and unambiguous than the commonly used chemical techniques. Aspects of the introduction of DMSO to rat kidney by perfusion through the vasculature are studied. C4S NMR imaging is applied to two common blood removal techniques and is shown to detect blood remaining in the vasculature after these washout procedures. A new method of completely removing blood from rat kidneys is shown to be more effective. Complete blood removal is demonstrated to be essential for homogeneous equilibration of tissues with a cryoprotective solution. Blood clots preventing the equilibration of DMSO in a kidney indirectly cause severe freezing damage. Equilibration of DMSO with rat kidney via perfusion with DMSO in University of Wisconsin (OW) solution is shown to cause minimal damage and proceed most quickly at 20°C. 3P1 NMR spectroscopy is performed on rat kidneys subjected to the new blood washout procedure and the relationship of the spectra to organ viability is discussed.
Item Metadata
Title |
NMR imaging and spectroscopy in cyrobiology
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1994
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Description |
Microscopic Chemical Shift Specific Slice Selective (C45) NMR imaging is
demonstrated to be a nondestructive and noninvasive technique for monitoring
the spatial distributions of solvents and of freezing/thawing phenomena.
Studies of test samples show that the C4S NMR imaging sequence can yield
quantitative maps of distributions of water., and dimethyl sulfoxide (DMSO).
The unique characteristics of C4S NMR imaging are potentially very useful in
studies of organ cryopreservation in which cryoprotective solvents (CPSs) like
DMSO successfully prevent freezing damage to cells in suspension but presently
cannot be used to preserve whole organs at subzero temperatures. There are no
other techniques available which are capable of monitoring CPS and water
concentration distributions as well as structural changes in organs during
cryopreservation procedures.
C4S NMR imaging is shown to be useful for monitoring the diffusion of
the cryoprotectant DMSO into rat kidney and liver tissues. A detailed
investigation of the possibility of mapping concentration distributions of
DMSO and water at various temperatures using images with short repetition
times verifies that DMSO and water in tissues can be quantified from image
signal intensities within certain concentration ranges. Diffusion rates are
easily obtained from the imaging data and are similar to those found in the
literature. A simple method for determination of effective diffusion
coefficients of DMSO in tissues is developed and shown to be more accurate and
unambiguous than the commonly used chemical techniques.
Aspects of the introduction of DMSO to rat kidney by perfusion through
the vasculature are studied. C4S NMR imaging is applied to two common blood
removal techniques and is shown to detect blood remaining in the vasculature after these washout procedures. A new method of completely removing blood
from rat kidneys is shown to be more effective. Complete blood removal is
demonstrated to be essential for homogeneous equilibration of tissues with a
cryoprotective solution. Blood clots preventing the equilibration of DMSO in
a kidney indirectly cause severe freezing damage. Equilibration of DMSO with
rat kidney via perfusion with DMSO in University of Wisconsin (OW) solution is
shown to cause minimal damage and proceed most quickly at 20°C. 3P1 NMR
spectroscopy is performed on rat kidneys subjected to the new blood washout
procedure and the relationship of the spectra to organ viability is discussed.
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Extent |
6931800 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-04-14
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0059650
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1994-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.