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UBC Theses and Dissertations
Adsorption of human serum albumin to polystyrene latex grafted with n,n- dimethylacrylamide Ramezani, Farhad
Abstract
Biocompatible materials can be defined as materials which can be introduced into a living organism without producing stress or traumatic response. When a solid material comes in contact with a biological medium, a layer of protein immediately binds to the surface. Subsequent defensive responses depend on the type and binding orientation of the protein on the surface. Therefore, primary surface protein binding is directly related to the type of physiological response observed in the body. Surfaces coated with grafted hydrophilic polymers can reduce non-specific protein adsorption. The hydrophilic nature of the grafted chain reduces the hydrophobic driving force. The long grafted chains extending out through the electrical double layer can reduce electrostatic driving forces. Non-specific protein can be excluded by the grafted chains and the solvent trapped between grafted chains. Polymer chains of hydrophilic poly(N,N -dimethylacrylamide) of different lengths were polymerized in situ by initiation from aldehyde groups on the surface of polystyrene latex. Using the techniques of particle electrophoresis, conductometric titration and elemental analysis, the surfaces of these latexes were analyzed. The adsorption isotherms of human serum albumin (HS A) to these latexes were measured. The results indicate that HSA has an affinity for the grafted poly(N,N -dimethylacrylamide). The exclusion effect of the grafted polymer in reducing HSA adsorption was greatest with the medium length grafted chains. The shorter or longer grafted chains on the latex did not reduce HSA adsorption compared with non-grafted latex.
Item Metadata
Title |
Adsorption of human serum albumin to polystyrene latex grafted with n,n- dimethylacrylamide
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1999
|
Description |
Biocompatible materials can be defined as materials which can be introduced into a
living organism without producing stress or traumatic response. When a solid material
comes in contact with a biological medium, a layer of protein immediately binds to the
surface. Subsequent defensive responses depend on the type and binding orientation of the
protein on the surface. Therefore, primary surface protein binding is directly related to the
type of physiological response observed in the body.
Surfaces coated with grafted hydrophilic polymers can reduce non-specific protein
adsorption. The hydrophilic nature of the grafted chain reduces the hydrophobic driving
force. The long grafted chains extending out through the electrical double layer can reduce
electrostatic driving forces. Non-specific protein can be excluded by the grafted chains and
the solvent trapped between grafted chains.
Polymer chains of hydrophilic poly(N,N -dimethylacrylamide) of different lengths
were polymerized in situ by initiation from aldehyde groups on the surface of polystyrene
latex. Using the techniques of particle electrophoresis, conductometric titration and
elemental analysis, the surfaces of these latexes were analyzed. The adsorption isotherms
of human serum albumin (HS A) to these latexes were measured. The results indicate that
HSA has an affinity for the grafted poly(N,N -dimethylacrylamide). The exclusion effect of
the grafted polymer in reducing HSA adsorption was greatest with the medium length
grafted chains. The shorter or longer grafted chains on the latex did not reduce HSA
adsorption compared with non-grafted latex.
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Extent |
3319973 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-06-16
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0089042
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1999-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.