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Designing separation systems in capillary electrophoresis based on the fundamental physicochemical properties of analytes Britz-McKibbin, Philip
Abstract
This thesis strives to develop an integrative approach towards separation design in capillary electrophoresis (CE) based on the fundamental physicochemical properties of analytes. It is divided into four main sections to deal with four important aspects of separation design in CE which include theory, selectivity, assay development and sensitivity. The use of additives in CE represents one of the most powerful ways to separate analytes in a mixture, since both electric field (mobility) and equilibria (affinity) are used in the separation process. Section A developed a theory to describe the migration behaviour of analytes in CE when using additives in the run buffer. The mobility of an analyte can be predicted at any additive concentration, capillary length, or voltage for rapid and systematic optimization of separation conditions. Section B involved the synthesis, characterization and application of TESMR, a water-soluble aromatic based macrocycle, as a new type of additive in CE. Separation of neutral species is accomplished by imparting an electrophoretic mobility to the analytes via differential complexation with TESMR. The development and validation of two different quantitative assays by CE is presented in Section C. A simple and sensitive method to analyze the γ-carboxyglutamic acid content of protein, urine and plasma is developed using CE with laser-induced fluorescence detection. The second assay involved the development of a robust method for the quantification of epinephrine in fifteen different dental anesthetic solutions. Selective on-line focusing of analytes using a discontinuous electrolyte system is presented in Section D as a facile way to improve concentration sensitivity in CE when using UV detection. Large volumes of a dilute sample can be focused into extremely sharp zones by carefully selecting appropriate sample and background electrolyte conditions. A summary of the results of the thesis and possible future endeavours is also discussed, as well as an integrative framework towards separation design in CE.
Item Metadata
Title |
Designing separation systems in capillary electrophoresis based on the fundamental physicochemical properties of analytes
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1999
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Description |
This thesis strives to develop an integrative approach towards separation design in
capillary electrophoresis (CE) based on the fundamental physicochemical properties of
analytes. It is divided into four main sections to deal with four important aspects of
separation design in CE which include theory, selectivity, assay development and sensitivity.
The use of additives in CE represents one of the most powerful ways to separate analytes
in a mixture, since both electric field (mobility) and equilibria (affinity) are used in the
separation process. Section A developed a theory to describe the migration behaviour of
analytes in CE when using additives in the run buffer. The mobility of an analyte can be
predicted at any additive concentration, capillary length, or voltage for rapid and systematic
optimization of separation conditions. Section B involved the synthesis, characterization and
application of TESMR, a water-soluble aromatic based macrocycle, as a new type of additive
in CE. Separation of neutral species is accomplished by imparting an electrophoretic
mobility to the analytes via differential complexation with TESMR. The development and
validation of two different quantitative assays by CE is presented in Section C. A simple and
sensitive method to analyze the γ-carboxyglutamic acid content of protein, urine and plasma
is developed using CE with laser-induced fluorescence detection. The second assay involved
the development of a robust method for the quantification of epinephrine in fifteen different
dental anesthetic solutions. Selective on-line focusing of analytes using a discontinuous
electrolyte system is presented in Section D as a facile way to improve concentration
sensitivity in CE when using UV detection. Large volumes of a dilute sample can be focused
into extremely sharp zones by carefully selecting appropriate sample and background
electrolyte conditions. A summary of the results of the thesis and possible future endeavours
is also discussed, as well as an integrative framework towards separation design in CE.
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Extent |
10635834 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-07-02
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0061524
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1999-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.