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Mechanistic studies on UDP-N-acetylglucosamine 2-epimerase

University of British Columbia

The bacterial enzyme UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2- epimerase) catalyzes the interconversion between the sugar nucleotide UDP-N-acetylglucosamine (UDP-GlcNAc) and its C-2" epimer UDP-N-acetylmarinbsamine (UDP-ManNAc). This enzyme differs from known racemases and epimerases in that it must invert a stereogenic center that bears a non-acidic proton. It must therefore employ a mechanism that is more complicated than a simple inversion by deprotonation followed by reprotonation on the opposite face of the reaction center. Previous studies on the epimerase obtained from natural sources have revealed that the substrate (UDP-GlcNAc) is required as an activator in the reverse reaction (UDP-ManNAc to UDP-GlcNAc), and that the enzyme is highly specific for its normal substrates. In addition, evidence was obtained suggesting that a proton transfer was ultimately responsible for the epimerization. A mechanism was suggested in which the epimerization was facilitated by increasing the acidity of the C-2" proton via transient formation of a sugar nucleotide ketointermediate (Kawamura et al., 1978: 1979; Salo, 1976). These results have been corroborated on a recombinant E. coli UDP-GlcNAc 2-epimerase and evidence supporting an alternate mechanism has been obtained. A substrate labeled with 18O at the anomeric position was prepared in order to be used in Positional Isotope Exchange (PIX) experiments. These experiments test whether the anomeric bond is cleaved during the epimerization. The scrambling of an 18O label located at the β- 31 phosphate moiety of UDP-epimers was detected by 31P NMR spectroscopy. This provided strong evidence for a mechanism involving cleavage of the anomeric bond and formation of 2- acetamidoglucal and UDP as intermediates. Additional studies with alternative substrates were used to gain more information about the mechanism of the epimerase. A 3'-deoxy analog (3'-deoxy-UDP-GlcNAc) was prepared and incubated with the epimerase to test for mechanisms involving the formation of a keto-intermediate at this center. The absence of epimerization with this substrate of the epimerase failed to rule out any such mechanisms, but suggested the need of the 3"-hydroxyl group as a necessary element for the recognition of the substrate. A trifluoroacetamido analog (UDP-GlCNAC- F3) was also prepared and proved to be an alternative substrate of the enzyme with a kcat of 2.6 x 10-2 s-1 an apparent Km of 3.40 ± 0.37 mM and a Hill coefficient of 1.6 ± 0.2. This compound was also an acceptable substrate for two sugar transferases. The possible involvement of neighboring group participation of the acetamido group during the epimerization was also tested by incubation of the oxazoline of ManNAc with the epimerase under conditions that permitted the detection of enzyme catalyzed formation of 2- acetamidoglucal or sugar nucleotides from this putative intermediate. The negative result obtained in this experiment along with the behavior of the epimerase with the trifluoroacetamido analog suggests (but does not prove) that neighboring group participation is not involved in the epimerization. The minimal description of the mechanism of the epimerase is unprecedented and involves the anti elimination of UDP from UDP-GlcNAc (syn from UDP-ManNAc) to form 2- acetamidoglucal and UDP as reaction intermediates followed by a syn addition (anti if started from UDP-ManNAc) of UDP to the double bond of the glycal intermediate to generate the sugar nucleotide epimer. Precedents for this process as well as a more detailed discussion of the type of elimination involved and future directions are also presented. [Scientific formulae used in this abstract could not be reproduced.]

Thesis/Dissertation

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2009-07-02

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http://hdl.handle.net/2429/9978

Chemistry

University of British Columbia

UBCV

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